Composite

Part:BBa_K4192121:Design

Designed by: Muhua Liu   Group: iGEM22_CAU_China   (2022-09-30)


Plac-obcA-obcB-Plac-Fpoar, Oxalate secretion gene circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2735
    Illegal PstI site found at 397
    Illegal PstI site found at 2878
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2735
    Illegal PstI site found at 397
    Illegal PstI site found at 2878
    Illegal NotI site found at 2330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2735
    Illegal BglII site found at 1541
    Illegal BglII site found at 3738
    Illegal XhoI site found at 304
    Illegal XhoI site found at 1428
    Illegal XhoI site found at 3550
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2735
    Illegal PstI site found at 397
    Illegal PstI site found at 2878
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2735
    Illegal PstI site found at 397
    Illegal PstI site found at 2878
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 720
    Illegal NgoMIV site found at 748
    Illegal NgoMIV site found at 784
    Illegal NgoMIV site found at 1014
    Illegal NgoMIV site found at 1791
    Illegal NgoMIV site found at 2208
    Illegal AgeI site found at 326
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the second Plac was added into the sequence by Gibson-Insert, and it was way too short for that enzymatic reactions. So we firstly connect it with obcB by overlap


Source

It mainly comes from genomic sequence of Burkholderia glumae and Fomitopsis palustris. The plasmid vector we used were pBBR1MCS2.

References